Microsoft Word - Yoshida10-03proof.doc

PKCδ (Fig. 3D). Taken together with the results of Fig.3B and C, early-phase JNK activation is, at least partly, requisite for the cytotox- icity of 4-HNE through PKCδ activation. Figure 3: PKCδ involvement [...] transient phosphorylation of PKCδ 5 min after exposure to 4-HNE, with a gradual decrease until 30 min (Fig. 3A). Next, our examination of whether the inhibition of PKCδ activity and function counteracts the cytotoxic [...] PKCδ inhibitor, significantly re- duced 4-HNE-induced cell damage in a dose-dependent manner (Fig. 3B and C). Collectively, these results support the con- cept that 4-HNE induces endothelial cell injury …