eldorado.tu-dortmund.de/server/api/core/bitstreams/90a5a43a-fc46-42d3-8af8-ed9f521d67aa/content
Original Article
tion After apoptosis induction, cells were lysed in ELB buffer (140 mmmol/L NaCl, 0.5% NP40, 50 mmol/L Tris-HCl, pH8.0, plus protease and phosphatase inhibitors) at a final concentration of 1×106 cells/ml [...] ion. As shown in Figure 2, after H2O2 (0.5 mmol/L) treatment for 3 h, the ratio of PS positive cells in vector group (pcDNA 3.1 ) significantly increased (from
0.33% to 6.78% ), while in αB-crystallin [...] cells (pcDNA 3.1-αB ), the ratio just slightly increased (from 0.23% to 0.96%), suggesting αB-crystallin could obviously prevent hydrogen peroxide induced apoptosis in the early stage.
3. αB-crystallin …