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Fmoc-NH-aa-COOH,4±8eqDIPEAin DMF, 2h, rt; for non-naturalaa:3±4eq.PyClocK,3eq.Fmoc-NH-aa-COOH, 4±6eq.DIPEAin DMF, 2h, rt; c) 10mol%1stgen.Grubbscatalystin DCE,3x 1h, rt; d) 4eq.Ac2O,8eq.DIPEA, DMF, 45min [...] andthescrambledpeptide6
(KD = 8.1± 0.1μM). (Fig3) Additionally, thestapledpeptide4 isableto induceamobility shift of DNA atalowerconcentrationthantheunstapledpeptide5 (S6-S8Figsin S1File).However, theFP [...] (4Â-pentenyl)alanine(S5),(R)-N-Fmoc-2-(4 Â-pentenyl)alanine(R5),(R)-N-Fmoc-2-(7Âoctenyl)alanine) (R8)at therespectivestapling positions.All peptideswerepreparedbearingaC-terminalamideon Rink amideor MBHA …