eldorado.tu-dortmund.de/server/api/core/bitstreams/b1d94352-0c33-46a9-a2c3-f9fd915a8d06/content
Catalytic Promiscuity of cGAS: A Facile Enzymatic Synthesis of 2′‐3′‐Linked Cyclic Dinucleotides
2-NH2-PuTP ATP 22 51[c]
8 6-S-GTP ATP 20 40[c]
9 7-CH-ATP GTP 27 88[c]
10 8-Br-ATP GTP 43 57 11 8-Br-dATP GTP 40 68 12 8-Br-GTP ATP 136 85[c]
13 8-Cl-ATP GTP 2 26 14 8-NH2-ATP GTP 10 27[c]
15 8-N3-ATP GTP 14 46 [...] 2’-F- ATP, 8-Br-ATP, 8-Cl-ATP, 8-NH2-ATP, and 8-N3-ATP with GTP into the corresponding cyclic dinucleotide derivative were carried out. Thus, 10 μmol of the substrates (5.8 mg 2’-F-ATP, 5.8 mg 8- Br-ATP, [...] resulted in 65% conversion of GTP with 2’-F-ATP, 47% GTP with 8-Br-ATP, 35% GTP with 8-Cl-ATP, 55% GTP with 8- NH2-ATP, and 58% GTP with 8-N3-ATP. These conversions were similar, but not fully comparable …
