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IDIOPATHIC PULMONARY FIBROSIS (IPF) is a disease characterized by progressive pulmonary insufficienc
150 mM NaCl, 2 mM EDTA, 1% Nonident P-40, 1% sodium deoxycholate, 0.1% sodium dodecylsulfate (SDS), 1 mM phenylmethylsulfonylfluoride, 0.04 TIU/µl aprotinin, 1 mM sodium orthovanadate and 50 mM sodium fluoride [...] Oligo(dT)12-18 (Invitrogen, Carlsbad, CA), 1× First strand Buffer (Invitrogen), 10 mM dithiothreitol, 0.5 mM deoxynucleotide triphosphates (dNTPs) and 5 U/ml SuperScript™ III (Invitrogen).
Polymerase chain [...] were by ANOVA with Bonferroni/Dunn’s tests used for post hoc analyses. Significance was accepted p <0.01.
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RESULTS
IL-10 inhibits TGF-β-induced COL1 mRNA expression in WI-38 cells. We previously reported …
